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Sudarsono
Departemen Agronomi Dan Hortikultura Fakultas Pertanian, Institut Pertanian Bogor
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Journal : Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal

 1 
Seleksi In Vitro Embrio Somatik Kacang Tanah pada Medium dengan Polietilen Glikol untuk Simulasi Kondisi Cekaman Kekeringan Enni Suwarsi Rahayu; Satriyas Ilyas; Sudarsono Sudarsono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.141

Abstract

This study was aimed to evaluate the effectiveness of in vitro selection for identifying PEG insensitive somaclonal variant of peanut somatic embryos (SE). In one of the experiments,  evaluation on responses of four peanut cultivars against selective medium containing polyethylene glycol 6000 (PEG 6000) was conducted and sub-lethal concentration of PEG was determined. In the other experiment, in vitro selection on selective medium containing sub-lethal level of PEG was conducted to identify PEG insensitive SE of peanut. Secondary SE of peanut cv. Badak, Kelinci, Singa, and Zebra were cultured on liquid MS medium supplemented with 16 µM of picloram and 5%, 10%, 15%, or 20% of PEG 6000. Survival of explant, average number of proliferated SE/explant, and total number of proliferated SE after in vitro selection were recorded monthly, up to three months. Sub-lethal level of PEG was defined as one inhibiting more than 95% of the total number of proliferated SE. In vitro selection on medium supplemented with sub-lethal level of PEG 6000 was conducted on at least 4000-5000 SE of peanut cv. Kelinci and Singa. The PEG insensitive SE was identified after subsequent three months of in vitro selection. The results showed that supplementation of PEG 6000 on medium for induction of SE inhibited proliferation of peanut SE. Sub-lethal level was obtained at 15% concentration of PEG 6000. The frequencies of obtaining PEG insensitive SE of peanut cv. Kelinci was 8% to 10%  and for peanut cv. Singa was 10% to 12%. The R0 plants of peanut cv. Kelinci (62 R0 plants) and Singa (48 R0 plants) regenerated from PEG insensitive SE were obtained and grown in the glasshouse to produce R1 and R2 seeds.
Co-Authors , Agustiansyah , Rustikawati , Sukendah , Triadiati , Witjaksono ABDUL RACHMAD BUDIONO Ade Wachjar Adisyahputra Adisyahputra, Adisyahputra Agung Endro Nugroho Agus Purwito Agus Rachmat AHMAD RIDUAN Ahmad Surkati Ali Husni Amarillis, Shandra Amarullah, Muhamad Roffi Andrie Al Kausar Anita Yuliyanti Aniversari Apriana Anneke Pesik Anwar, Aswaldi Aprizal Zainal Arfan Nazhri Simamora Arief Hidayat Aris Munandar Asep Setiawan ASMONO, DWI Atmitri Sisharmini Bambang Heliyanto Bambang S. Purwoko BAMBANG SUGIRI Barahima Abbas C J Soegihardjo Cartealy, Imam Civi Cartealy, Imam Civi Catur Herison Cecep Kusmana Christoph Leuschner Clarry Sada Colleen M. Higgins, Colleen M. Darmawan Saptadi Deka Rotama Dewi Sukma Didiek Hadjar Goenadi Diky Anggoro DINARTY, DINY Dini Dinarty, Dini DINI NURDIANI Diny Dinarti Djoko Santoso DWI ANDREAS SANTOSA Dwi Asmono DWI HAPSORO Dwi Hapsoro Edi Guhardja Efendi, Darda Elina, Juanita ELSJE T. TENDA, ELSJE T. Endang Pudjihartati Endrizal Eneng Fakhrunnisa Enni Suwarsi Rahayu Erick Raynalta, Erick Fajar Laksono Suroso Fakhrunnisa, Eneng Fatsan, Ahmad FETRINA OKTAVIA Germini Alam Giyanto . Gusti Ayu Kade Sutariati H. M.H. Bintoro Djoefrie HAJRIAL ASWIDINNOOR Harsini ,, Harsini Hartati, Raden Roro Sri Haryadi Permana Hasriadi Mat Akin Hengky Novarianto Hengky Novarianto Hidayat Pawitan Hiroshi Ehara Hugo Volkaert, Hugo I MADE ARTIKA IBNUL QAYIM IDA HANARIDA SOMANTRI Iim Fatimah Ika Mariska Ikhsanudin Ikhsanudin Irman Firmansyah Ismail Maskromo Ismail Maskromo Iwa Sutardjo, Iwa Iwan Setiawan JAJAH KOSWARA Juang Gema Kartika Jumanto Jumanto Jumanto Jumanto, Jumanto Khoerul Anwar Khursatul Munibah Kukuh Murtilaksono Kukuh Setiawan Kurniasari, Praharisti Kurniawan R. Trijatmiko Kusumo Nugroho Kuswanhadi Kuswanhadi, Kuswanhadi Laksmita Prima Santi Laksono Trisnantoro Lila Yuwana Lollie Agustina P. Putri M. Herman Marbelisa Briliani Mardikaningsih, Agusti Marenda Ishak S MASKROMO, ISMAIL MEITY A. TULALO, MEITY A. Memen Surahman Meynarti Sari Dewi Ibrahim Muchammad Ali Safaat Muhammad Hasyim Bintoro Muhammad Herman MUHAMMAD HERMAN Muhammad Machmud Muhammad Machmud Mutia Dewi Yuniati Neviana Wahyuni Nihayati, Hanik Endang Niniet Indah Arvitrida Noor Cahaya NOVARIANTO, HENGKY Nur Khayati Nurhajati A. Mattjik Nursalam Nursalam Nurul Khumaida Paulus B.K. Santoso Purwaningsih Purwaningsih purwoko, devit Purwoko, Devit R B Gobel Ralf G. Dietzgen, Ralf G. Reni Indrayanti ROEDHY POERWANTO Rr Sri Hartati Rr Sri Hartati, Rr Sri Rr. Sri Hartati Rubiyo Rubiyo RULLY DYAH PURWATI RUSMILAH SUSENO S. Arsyad S. Ilyas Said Harran Saiful Akhyar Lubis SATRIYAS ILYAS Satriyas Ilyas Satya Nugroho Shinta Hadiyantina Sholeh Avivi Siswanto Siswanto Siti Mufidah Siti Sunarintyas SOEKISMAN TJITROSEMITO Sri H. Hidayat Sri H. Hidayat Sri H. Hidayat Sri Indarto Sri S. Harjadi Sri Soenarsih DAS Stephanie Wilamarta Sudari, Sudari Sudibyo Martono Sudirman Yahya SUDJINDRO SUDJINDRO Sugiyanta Sukendah S, Sukendah SUKENDAH SUKENDAH, SUKENDAH Sumali Wiryowidagdo Suryo Wiyono Susilo Indrawati Swastika, Maria Syafaruddin Syafaruddin TENDA, ELSJE T. Teuku Tajuddin TINCHE, nFn Tri J. Santoso Tri J. Santoso Tri Joko Santoso Tri Joko Santoso U.S WIRADISASTRA UJANG KHAIRUL Urai Salam Visia Assyafira Suwarto vyta andri setyo utami Wening Enggarini Widiatmaka . Widiatmaka Widiatmaka Widodo Widodo Wiwin Ambarwulan Yudiwanti Wahyu E. K. Yulis Setiya Dewi Yundari, Yundari Yuni Wibowo Yusnita Yusnita